Our group has been working for several years on the modification of proteins by electrophilic lipids (protein lipoxidation), which is increased in pathological situations associated with oxidative stress and can drastically alter protein function. We have identified targets of lipoxidation and characterized the structural and functional consequences of the modification. The major constituents of the cytoskeleton, including actin, tubulin and vimentin, are targets for these modifications. In some cases, characterization of their modification has led to discern the important role of the target residues in the regulation of protein function under basal and stress conditions. We are interested in exploring new mechanisms of regulation of cytoskeletal proteins, mainly in response to electrophilic species, with a focus on intermediate filaments. We are very much interested in broadening our view of this field and incorporating new methodologies.
Our group employs molecular and cellular biology techniques. We mainly employ cultured cells transfected with expression constructs of the proteins of interest that are monitored by fluorescence microcopy or used for various cellular assays. We have some experience in basic proteomic techniques. We also carry out in vitro assays with recombinant proteins. More detailed information can be gathered from our publications. We are happy to share this expertise with other participants.
We are very much interested in the techniques considered in WG2 and WG4, in particular, single particle cryo-EM, cellular cryo-tomography, super-resolution and volume imaging techniques, mass spectrometry techniques and gene editing. Therefore, although we think that our work may fit better in WG1, in particular in Task 2 (“Study the roles of different cellular components in regulating the mechano-biological properties of cells by looking at the nucleoskeleton, cytoskeleton, cell adhesion and extracellular matrix components that are involved in mechano stability and mechano ignaling and using biophysical, genetics and cell biology approaches”) we are also very interested in the actions of the other groups.
Amoxicillin haptenates intracellular proteins that can be transported in exosomes to target cells.
Sánchez-Gómez FJ, González-Morena JM, Vida Y, Pérez-Inestrosa E, Blanca M, Torres MJ, Pérez-Sala D.
Allergy. 2016 Jun 20. doi: 10.1111/all.12958. [Epub ahead of print]
Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding.
Pérez-Sala D, Oeste CL, Martínez AE, Carrasco MJ, Garzón B, Cañada FJ.
Nat Commun. 2015 Jun 2;6:7287. doi: 10.1038/ncomms8287.
An isoprenylation and palmitoylation motif promotes intraluminal vesicle delivery of proteins in cells from distant species.
Oeste CL, Pinar M, Schink KO, Martínez-Turrión J, Stenmark H, Peñalva MA, Pérez-Sala D.
PLoS One. 2014 Sep 10;9(9):e107190. doi: 10.1371/journal.pone.0107190.
Modification of cysteine residues by cyclopentenone prostaglandins: interplay with redox regulation of protein function.
Oeste CL, Pérez-Sala D.
Mass Spectrom Rev. 2014 Mar-Apr;33(2):110-25. doi: 10.1002/mas.21383. Review.
Identification of aldo-keto reductase AKR1B10 as a selective target for modification and inhibition by prostaglandin A(1): implications for antitumoral activity.
Díez-Dacal B, Gayarre J, Gharbi S, Timms JF, Coderch C, Gago F, Pérez-Sala D.
Cancer Res. 2011 Jun 15;71(12):4161-71. doi: 10.1158/0008-5472.CAN-10-3816.
9-11 September 2019, Trnava, Slovakia