Aswendt Markus

Country: Germany
Laboratory webpage
E-mail: markus.aswendt@uk-koeln.de

Participation in Working Groups

  • WG4 - Mechanobiological principles of rare and common diseases

Research Interests

My research is focused on the combination of innovative methods to visualize and influence plasticity and regenerative processes in pre-clinical mouse models of stroke. I have developed an atlas-based brain mapping approach for the macro- and microscopic scale combining complementary techniques and extensive image post-processing to identify mechanisms of plasticity and regeneration underlying brain network changes which correlate with sensorimotor recovery after stroke. As there is no therapy available to date which targets recovery directly, the aim of my ongoing experiments is to establish novel stimulation paradigms which selectively stimulate recovery-related brain networks.

Technologies offered to other EuroCellNet participants

  • stroke model (photothrombosis)
  • small animal neuroimaging (MRI: rs-fMRI, DTI, T1, T2)
  • intracerebral (viral) injections
  • behavior tests (rotating beam, grid walk, cylinder test)
  • software for MRI processing, registration of MRI and histology with the Allen Mouse Brain Atlas
  • database for managing preclinical animal research data

Technologies sought from other EuroCellNet participants

  • cell culture models (astrocytes, neurons)
  • histology and in-situ hypdridization
  • (high-throughput) fluorescence microscopy and slide scanner
  • RNA sequencing

Publications

1. Pallast N, Diedenhofen M, Blaschke S, Wieters F, Wiedermann D, Hoehn M, Fink GR, Aswendt M.
Processing pipeline for Atlas-based Imaging Data Analysis of structural and functional Mouse Brain MRI (AIDAmri)
Frontiers in Neuroinformatics (IF 3.1) 2019; 13:42. https://doi.org/10.3389/fninf.2019.00042

2. Vogel S, Schäfer C, Hess S, Minassian A, Schwarz M, Folz-Donahue K, Kukat C, Ehrlich M, Zaehres H, Kloppenburg P, Hoehn M, Aswendt M.
The in vivo timeline of human neural stem cell differentiation
Stem Cell Research, Volume 37, May 2019, 101429. https://doi.org/10.1016/j.scr.2019.101429

3. Ito M*, Aswendt M*, Lee AG, Ishizaka S, Wang EH, Levy SL, Smerin D, Leuze C, Goubran M, Cheng MY, Steinberg GK.
RNA-Sequencing Analysis Revealed a Distinct Motor Cortex Transcriptome in Spontaneously Recovered Mice After Stroke.
Stroke 2018; 49:2191-2199. https://doi.org/10.1161/STROKEAHA.118.021508

4. Adamczak J*, Aswendt M*, Kreutzer C, Rotheneichner P, Riou A, Selt M, Beyrau A, Uhlenküken U, Diedenhofen M, Nelles M, Aigner L, Couillard-Despres S, Hoehn M.
Neurogenesis upregulation on the healthy hemisphere after stroke enhances compensation for age-dependent decrease of basal neurogenesis.
Neurobiology of Disease (2017; 99:47-57. https://doi.org/10.1016/j.nbd.2016.12.015

5. Aswendt M, Gianolio E, Pariani G, Napolitano R, Fedeli F, Himmelreich U, Aime S, Hoehn M.
In vivo imaging of inhibitory, GABAergic neurons by MRI.
NeuroImage 2012, 62(3):1685-1693.